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Fluorescence anisotropy measurements in solution: Methods and reference materials (IUPAC Technical Report)

M. Ameloot, M. vandeVen, A. U. Acuna and B. Valeur Pure Appl. Chem., 2013, 85, 3, 589-608

After recalling the basic relations relevant to both steady-state and time-resolved fluorescence polarization, it is shown how the values of steady-state polarized intensities recorded experimentally usually need to be corrected for systematic effects and errors, caused by instrumentation and sample properties. A list of selected reference values of steady-state fluorescence anisotropy and polarization is given. Attention is also paid to analysis of time-resolved fluorescence anisotropy data obtained by pulse fluorometry or phase and modulation fluorometry techniques. Recommendations for checking the accuracy of measurements are provided together with a list of selected time-resolved fluorescence anisotropy data as reported in the literature.

Type :

Publication

Dates :

Paru le 11 janvier 2013

Informations complémentaires :

doi 10.1351/pac-rep-11-11-12