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Improved Photon Yield from a Green Dye with a Reducing and Oxidizing System

A. L. Gall, D. Dulin, G. Clavier, R. Méallet-Renault, P. Bouyer, K. Perronet and N. Westbrook ChemPhysChem, 2011, 12, 9, 1657-1660

An optimized chemistry turns a small green dye into a useful label for single-molecule experiments where steric hindrance is an issue. Thanks to a reducing and oxidizing system combined to oxygen depletion, a single Bodipy (boron-dipyrromethene) FL fluorophore emits, on average, 20 times more photons around 510 nm (see picture) and its lifetime before photobleaching is increased by the same amount, reaching several seconds.

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Publication

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Paru le 31 mai 2011

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DOI: 10.1002/cphc.201100085