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Rhodamine B nanocrystals: elaborations, characterizations and functionalizations for biosensing applications

Dubuisson, E; Marty, L; Cattoen, X; Pansu, R; Szunerits, S; Ibanez, A JOURNAL OF SOL-GEL SCIENCE AND TECHNOLOGY 2014, 72, 179-188.

A biological sensor based on fluorescent organic nanocrystals (NCs) of Rhodamine B grown in sol-gel thin films was developed. The original signalization function is based on fluorescence contrasts of NCs, which exhibit a simple fluorescence signature, good photostability and higher fluorescence intensities compared to dispersed dye molecules. Thanks to a well-controlled dissolution process of the sol-gel surface, accurately followed by atomic force microscopy, the NCs were made emerging just a few nanometers above the silicate thin films to be directly accessible to biological macromolecules. Thus, hairpin-shaped DNA, functionalized by a probe-molecule (DNA probe), has been grafted onto nanocrystal surfaces leading to a fluorescence quenching by Forster resonance energy transfer. After hybridization of these hairpin-shaped DNA probes with their complementary DNA-target, the molecular probes and NCs are pulled apart, stopping thus the quenching. This "turn-on" of nanocrystal fluorescence allows thus a label-free DNA detection. The preparation methodology of the signalization function, its functionalization by hairpin-shaped DNA probes and first DNA-sensor experiments are presented.

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Publication

Dates :

Paru le 8 juillet 2014

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DOI: 10.1007/s10971-014-3431-4